May it be under routine testing or alternative media, especially for unculturable species to grow, what are the certain updates to these?
this is a good place to start
Article Strategies for culture of ‘Unculturable’ bacteria
This study by Santo et al. (2022) confirms the possibility of using whey (a dairy industry residue) culture media as an alternative to obtaining bacterial spores (of Bacillus atrophaeus).
Article Growth, development and thermal resistance of Bacillus atrop...
Since culture media can be expensive, it is important to identify alternative options or reduce the amount of agar used in laboratories with limited resources. In this regard, Arnan-Parh et al. have investigated the use of cowpea as an affordable alternative culture medium. Additionally, some studies have utilized starch or vegetables as a cheaper growth medium for bacteria and fungi. This study seeks to replace the nutrient source with a protein formulation using locally available, cost-effective legume seeds such as green gram, black gram, cowpea, and soya meat. These seeds are rich in protein and can serve as a viable substitute for traditional nutrient sources in culture media preparation.
Reference: Arulanantham, R., Pathmanathan, S., Ravimannan, N., & Niranjan, K. (2012). Alternative culture media for bacterial growth using different formulation of protein sources. Scholars Research Library. https://www.scholarsresearchlibrary.com/articles/alternative-culture-media-for-bacterial-growth-using-different-formulation-ofprotein-sources.pdf
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