Can any one explain or send a standard protocol for Ulvan Polysacharide extraction from sea weed/algae ?

One of my student is interested in studying the Prebiotic application of ulvan polysaccharide. She extracted polysaccharide using hot water extraction and ethanol precipitation (Following an...

02 September 2020 3,530 3 View

How to use Sephadex Column to isolate aminoglycoside compounds?

There are several types of Sephadex and they can be used in several manners for different compounds. I want to use Sephadex column to isolate aminoglycoside. If anyone having idea, how to use...

21 April 2020 2,478 4 View

Why am I getting the higher COD after applying AOP (O3+ H2O2)?

I am treating the industrial effluent by AOP using H2O2+ O3. I am getting higher COD values than raw wastewater itself. I quenched the H2O2 by adding MnO2. Samples were filtered 0.2-micrometer...

24 November 2019 3,117 8 View

The solubility of Acetylated paramomycin salt is in H2O only. How to isolate Acetylated Paramomycin salt synthesized from Paramomycin Sulfate salt?

Column chromatography is the technique to isolate the organic compound which is soluble in organic solvent. And I want to isolate a compound which is soluble only in water. Any technique or...

20 October 2019 9,207 1 View

Is it possible to find the sensitivity of RMSD and RMSF in MD simulation through gromacs ?

Is it possible to find the regions of the protein structures most susceptible to temperature impact in terms of RMSD and RMSF , during MD simulation ?

06 May 2018 8,855 3 View

What is the normal doubling time for U87 cells? I have never worked with them before and I feel my cells are growing very slowly.

30 May 2016 8,471 3 View

How do I design primers for protein isoforms?

16 June 2015 7,751 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View