One of my student is interested in studying the Prebiotic application of ulvan polysaccharide. She extracted polysaccharide using hot water extraction and ethanol precipitation (Following an article - Ulvan polysaccharide was extracted by the modified procedure of Hussein et al. using the methods of hot water-extraction and ethanol-precipitation. 100 g of dried Ulva lactuca were extracted three times at 100°C for 2 h with double-distilled water. The collected solution was centrifuged at room temperature for 15 minutes at 6000 rpm; then the extract was precipitated by ethanol (4-times the used volume of aqueous extract), and this mixture was kept at 4°C overnight. The precipitate was collected by centrifugation at 6000 rpm for 15 min and then dissolved in distilled water to dialyze against deionized water for 72 h for removing any traces for the alcohol. Finally, the precipitate was freeze-drying to yield the crude ulvan polysaccharide which its touch and appearance were like a brown gel. ). Next she wanted to do dialyze and and ion chromatography. For that she wanted to know the size of the dialyze bag and which absorbent is used for chromatography. Could any one give a standard protocol for extraction.... Would be very helpful
Ulvan: A systematic review of extraction, composition and ... https://www.sciencedirect.com/science/article/pii/S2211926418308373
Kindly check https://www.hindawi.com/journals/bmri/2010/525291/
Also check https://www.researchgate.net/publication/236972522_Characterization_of_ulvan_extracts_to_assess_the_effect_of_different_steps_in_the_extraction_procedure
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