Does anybody know how we can perform a blast searches of specific DNA sequence on the hela genome (and other specific cell lines). Many cell lines have been sequenced but it is not obvious these can be search by blast!
thanks
I think the HeLa genome is stored in DBGap, and can be downloaded. You can then make your own BLAST database from the FASTA sequence.
As I understand, the issue is that human cell line sequences are not laboratory artefacts, but the genomes of identifiable human beings. This brings special ethical concerns in releasing the data, so it is necessary to enter into a formal agreement before the data can be accessed.
thanks, i know the ethical issues of the hela genome sequence. I tought, it was possible to query the genome for a specifc sequence.
Hi,Didier,If you just want to check a mutation of your interested gene,maybe you can try this linkhttp://cancer.sanger.ac.uk/cell_lines/sample/overview?id=1298134 and click the mutation tag.I hope you can find the information you need.
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