Didier Fesquet

5 Questions 67 Answers 0 Followers

Questions related from Didier Fesquet

Looking for Myc or Tags-T2A-PURO/Blast vectors?

Hi all, I am looking for a vectors backbone containing an expression cassette for epitope tagging my candidates are -Puro/blast-T2A-3MYC (other tag will do ) -MYC-T2A-PURO/Blast (other tag will...

30 September 2019 6,007 1 View

Glucose deprivation experiment?

several authors report cell survival for 48h upon glucose depletion , in my hands after 15hours...100% cells death..Hela, U2OS..any hints to share.!

15 November 2016 4,411 7 View

looking for CRE recombinase expression vectors?

addgene is a great ressources for plasmid. Unfortunatly. They do not have an expression vector for CRE recombinase on a blasticidine or zeocin or hygro backbone (I am not interested by puromycine)...

20 May 2015 1,028 2 View

Are there any Blast searches on HeLa genome?

Does anybody know how we can perform a blast searches of specific DNA sequence on the hela genome (and other specific cell lines). Many cell lines have been sequenced but it is not obvious these...

27 November 2014 2,720 3 View

According to your experiences, what is the best cover slip mounting medium for IF?

I have tried prolong antifade, vectashield, home made moviol+antifade, but none of them give me entire satisfaction. Quite often, I observe either a green or red background where there are no...

07 December 2012 8,204 46 View