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Hi all, I am looking for a vectors backbone containing an expression cassette for epitope tagging my candidates are -Puro/blast-T2A-3MYC (other tag will do ) -MYC-T2A-PURO/Blast (other tag will...
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several authors report cell survival for 48h upon glucose depletion , in my hands after 15hours...100% cells death..Hela, U2OS..any hints to share.!
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addgene is a great ressources for plasmid. Unfortunatly. They do not have an expression vector for CRE recombinase on a blasticidine or zeocin or hygro backbone (I am not interested by puromycine)...
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