Are you interested to submit a paper in the special issue "Solar Radiation" in "Remote Sensing" ?

I you are interested to submit a paper in the special issue "Solar Radiation" in "Remote Sensing", please have a look on the 2 following files and/or on the web site :...

01 February 2021 2,954 3 View

Looking for Myc or Tags-T2A-PURO/Blast vectors?

Hi all, I am looking for a vectors backbone containing an expression cassette for epitope tagging my candidates are -Puro/blast-T2A-3MYC (other tag will do ) -MYC-T2A-PURO/Blast (other tag will...

29 September 2019 5,904 1 View

How to run seasonal trajectories using GUI HYSPLIT model

29 December 2016 9,477 9 View

To illustrate an exhibition devoted to insect bioluminescence, I am looking for living adults/larvae of Coleoptera Lampyridae. Can anyone help me ?

06 January 2015 2,403 6 View

Are there any Blast searches on HeLa genome?

Does anybody know how we can perform a blast searches of specific DNA sequence on the hela genome (and other specific cell lines). Many cell lines have been sequenced but it is not obvious these...

26 November 2014 2,634 3 View

Is anybody aware of a direct use of correspondence analysis in association rule learning (from pattern recognition & DB engineering view point)?

As the basic concepts used in association rule learning are related to conditional probability and ratio to independence, I was wondering if Correspondence Analysis has been used in the literature...

01 January 1970 5,480 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View