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Questions related from Arrhenius Lin
A 65 years old male patient, once a heavy smoker and HBV carrier suffers from some form of cancer with pericardial sac metastasis.The PET/CT shows there are four nodules with high SUVmax (higher...
30 September 2021 674 3 View
I have been trying to knockdown a gene of lysosome recently. When cells are transfected with pSuper-GFP-neo-target shRNA, the resulting transfectants seems hard to receive other plasmids for a...
29 May 2013 1,392 2 View
I have been trying to express some large eukaryotic proteins,over 100kDa in sizes, in BL21(DE3) and Rosetta(DE3), the outcome is frustrating.Does anybody have any ideas about how to achieve this goal?
30 March 2013 7,862 11 View
I have been working on transfecting HeLa cells with two expression vectors using Turbofect. One is pCI-Neo and the other is pEGFP. The transfection rate of pCI-Neo is quite low, however, in...
08 December 2012 4,863 20 View
A patient has no significant mutations in all available driving oncogene, and a PD-L1 negative state(Although it may be a false negative result from IHC).Worse still,he has pericardical...
01 January 1970 9,362 8 View
Does some gene mutation that increase migration necessarily increase invasion?Is it possible that some mutation that enhance cell migration but inhibit its invasion?
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