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Questions related from Arrhenius Lin
A 65 years old male patient, once a heavy smoker and HBV carrier suffers from some form of cancer with pericardial sac metastasis.The PET/CT shows there are four nodules with high SUVmax (higher...
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I have been trying to knockdown a gene of lysosome recently. When cells are transfected with pSuper-GFP-neo-target shRNA, the resulting transfectants seems hard to receive other plasmids for a...
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I have been trying to express some large eukaryotic proteins,over 100kDa in sizes, in BL21(DE3) and Rosetta(DE3), the outcome is frustrating.Does anybody have any ideas about how to achieve this goal?
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I have been working on transfecting HeLa cells with two expression vectors using Turbofect. One is pCI-Neo and the other is pEGFP. The transfection rate of pCI-Neo is quite low, however, in...
08 December 2012 4,921 20 View
A patient has no significant mutations in all available driving oncogene, and a PD-L1 negative state(Although it may be a false negative result from IHC).Worse still,he has pericardical...
01 January 1970 9,405 8 View
Does some gene mutation that increase migration necessarily increase invasion?Is it possible that some mutation that enhance cell migration but inhibit its invasion?
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