Arrhenius Lin

3 Questions 17 Answers 0 Followers

Questions related from Arrhenius Lin

Is there some transfection method that delivers plasmid in a endosomal-lysosomal independent pathway?

I have been trying to knockdown a gene of lysosome recently. When cells are transfected with pSuper-GFP-neo-target shRNA, the resulting transfectants seems hard to receive other plasmids for a...

29 May 2013 1,383 2 View

How to express large recombinant proteins with molecular weight over 100kDa in E.coli?

I have been trying to express some large eukaryotic proteins,over 100kDa in sizes, in BL21(DE3) and Rosetta(DE3), the outcome is frustrating.Does anybody have any ideas about how to achieve this goal?

30 March 2013 7,850 11 View

Can anyone suggest ways to optimize my co-transfection?

I have been working on transfecting HeLa cells with two expression vectors using Turbofect. One is pCI-Neo and the other is pEGFP. The transfection rate of pCI-Neo is quite low, however, in...

08 December 2012 4,854 20 View