It is known that tropomodulin is present in smooth muscle cells. It is not clear to me whether this capping protein caps the pointed-ends of actin filaments, as in striated myocytes.
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Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Qual o fator motivacional acerca da preparação dos professores, bem como ocorre a práxis educativa nas escolas. Em análise, o fazer educacional comtempla os ditames da educação contemporânea?
16 July 2024 8,652 3 View
I will polymerise Aniline on ITO glass WE, using 1M of H2SO4 and a potentiostat. However, I am not sure if I have to do the electrochemical polymerisation inside a hood. Is it necessary? Is the...
10 July 2024 2,937 3 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,178 2 View
What do you think is the main advantage of cell membrane-camouflaged nanoparticles, according to your experience?
04 July 2024 6,902 2 View
Does anyone have the full text of this article? KELIKIAN H, RIASHI E, GLEASON J. Restoration of quadriceps function in neglected tear of the patellar tendon. Surg Gynecol Obstet. 1957...
08 June 2024 7,054 0 View
If you prepare drug-loaded nanoparticles but only obtain microparticles (~2,000-4,000 nm), PDI~0.2, how can you reduce the size to reach ~100 nm?
02 June 2024 2,693 4 View
How to know if two drug molecules interact with each other, the strength (and type) of interaction and the possibility to form nanoparticles?
02 June 2024 4,304 1 View
Hello everyone, I am starting a soil mapping project in a specific crop region, and my goal is to perform shotgun sequencing (Illumina NovaSeq) to identify microorganisms and potential genes. This...
24 May 2024 5,021 4 View
As keys in input, I used: %Mem=20GB %Nproc=16 %chk=etilantraquinona_professor_func=6-31G_Au_Norte.chk #B3LYP/GEN OPT FREQ=RAMAN Nosymm PSEUDO=READ SCF=TIGHT" **** 0 2 . . . . C O H...
17 May 2024 391 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...
03 August 2024 835 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View