Some fluorescent dyes for example FITC and rhodamine are stated to be less stable in pH fluctuations. What precautions should be taken to study Actin cytoskeleton and what are the preferred dyes?
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
Hello, I have come across a paper where it is stated that Sequence identity between these two proteins is ~36%. I performed BLAST and in the summary section, it is stated that that percent...
16 January 2024 1,478 2 View
Hello, I am working on a protein that belongs to NUDIX family hydrolases and therefore requires Mg ion as a cofactor. But the protein that I have downloaded from PDB is an apoprotein. I want to...
11 January 2024 8,105 2 View
Dear all, I have developed a pharmacophore hypothesis using receptor cavity since my protein has no co-crystallized ligand. I , now, aim to validate it using a set of actives and decoys. I tried...
30 November 2023 1,424 0 View
Also, is there a way to convert SMILES into IUPAC or common name of the compound?
30 November 2023 304 2 View
I have a protein that binds to ATP and another nucleotide. I want to dock them so that I can find out the binding site residues in the protein. Which tool can I use?
10 October 2023 8,217 4 View
How to correct of desk reject of an article
08 October 2023 4,165 1 View
Is it possible to remove the titanium-based coating using any solvents/ acids or other chemicals from the steel without affecting the surface of the steel?
29 September 2023 8,427 1 View
I have a 3D-structure of a protein, but it is an apoprotein. I am using Maestro for molecular docking. I want to find out the protein binding site in my protein.
27 September 2023 1,748 7 View
I am currently working on phytoremediation. i want to know about calculations of making solution from lead acetate tri hydrate. the required amount of Pb in soil is 250mg/kg.
25 January 2023 6,035 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View