Your primers will last longer in buffered solution than water. I always suspend my master stock of primers at 100uM in 10mM Tris-HCL, pH 8.5. I then dilute these to 10uM stocks that I use in downstream applications like PCR.
Your primers will last longer in buffered solution than water. I always suspend my master stock of primers at 100uM in 10mM Tris-HCL, pH 8.5. I then dilute these to 10uM stocks that I use in downstream applications like PCR.
If water is used I think- The pH of water can be low (especially if it is DEPC treated) and this will be damaging to primer (DNA) over time.
This is what I found:
To resuspend/ reconstitute PRIMERS:
Use a buffered solution at neutral pH to protect from acid hydrolysis. EDTA (1 mM) in the master stock is also a good idea to protect against DNases and when you dilute the primers for working stocks, the EDTA will be sufficiently dilute that it will not interfere with Taq activity.
I have been using normal milliQ water and never had any problem. 10 mM Tris/HCl pH 8.5 might be better though.
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