Matteo Da Ros

3 Questions 10 Answers 0 Followers

Questions related from Matteo Da Ros

Does UV-crosslink also work between proteins? And how is it possible to "reverse" it?

UV-crosslink is widely used to bind together proteins and RNA for co-isolation. Does it works also to connect proteins with other proteins? Is it possible to "reverse" such crosslink? For example,...

28 June 2016 7,630 3 View

Can anyone suggest a protocol to isolate autophagosomes?

I am starting from single cell suspension and I am interested in isolating autophagosomes and maybe other vesicles (lysosomes, ER, mitochondria, MVBs). I don't have the instrumentation for...

02 March 2015 8,311 2 View

Does anyone know of some working antibodies against vesicular markers?

I am looking for antibodies against markers for: - early endosomes - late endosomes - autofagosomes - lysosomes - multi vesicular bodies I am working with mouse testis sections from formalin fixed...

04 March 2014 972 1 View