Alexander Rosenberg

2 Questions 4 Answers 0 Followers

Questions related from Alexander Rosenberg

What's the most efficient protocol to integrate a plasmid into the yeast genome?

I am looking to maximize the number of resulting colonies. How many colonies should I expect to get? Also how does efficiency change when targeting different genomic locations? Cheers, Alex

30 September 2014 4,871 4 View

How does permeabilization of fixed mammalian cells differ when using hydrochloric acid vs ethanol (or methanol)?

I noticed in the FISSEQ protocol (http://www.sciencemag.org/content/343/6177/1360.long) fixed cells are treated with hydrochloric acid after they have already been permeabilized with ethanol or...

10 September 2014 4,045 1 View