Dr. Godfred A. Menezes

8 Questions 69 Answers 0 Followers

Questions related from Dr. Godfred A. Menezes

Maintenance of PFGE electrophoresis cell during long breaks (not in use for say 3 months)!?

What is the usual recommended procedure for maintenance of the PFGE electrophoresis cell during long breaks (not in use for 3 months): 1. Clear all the buffer/water from tubings, OR 2. Flush once...

27 April 2016 3,001 7 View

Is anyone using the NEB Lambda ladder PFG Marker?

NEB Lambda ladder PFG Marker - literature says electrophoresis run is for 48 hrs. We have optimised our isolate plugs for 19 hrs. How do I synchronize it now? One issue is we get 12-13 bands...

02 November 2014 5,690 11 View

What are the prospectives of additives for synergism along with antibiotics against antibiotic resistant bacteria?

Synergism along with antibiotics against antibiotic resistant bacteria!

15 September 2014 8,812 8 View

Why am I not able to change/adjust the parameters (in. switch and fin. switch time and total run time) in the power module of CHEF DRIII?

Have you faced such a problem? If yes, please suggest how was the problem rectified. Its a new equipment, being used for the first time. Under programme block 1, the initial switch time is fixed...

04 September 2014 4,857 3 View

Are buffered solution at neutral pH better than water for resuspending the master stock PRIMERS?

Resuspending the master stock PRIMERS

25 August 2014 3,561 5 View

How can I overcome inconsistency for optimum chromosome spread in conventional cytogenetics?

Inconsistency of optimum chromosome spread.

18 August 2014 4,352 13 View

What is your view about hypnosis and meditation in medicine/ treatment?

What is your opinion of hypnosis and meditation in medicine/ treatment?

28 July 2014 1,487 22 View

Among PCR based bacterial typing methods which have you found quite promising?

PCR based typing methods such as- Ribotyping, RAPD etc.... For Enterobacteriaceae members I found ERIC-PCR to be quite good. Many Institutes do not have facilities for PFGE and MLST. Some...

23 July 2014 9,546 1 View