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Questions related from Dr. Godfred A. Menezes
What is the usual recommended procedure for maintenance of the PFGE electrophoresis cell during long breaks (not in use for 3 months): 1. Clear all the buffer/water from tubings, OR 2. Flush once...
27 April 2016 3,090 7 View
What is the easiest and fastest way to isolate antimicrobial metabolite from unknown fungus (grown from plants)?
10 February 2015 1,204 3 View
Teixobactin was discovered using a new method of culturing bacteria in soil, which allowed researchers to grow a previously unculturable bacteria now named Eleftheria terrae, which produces the...
25 January 2015 6,964 11 View
NEB Lambda ladder PFG Marker - literature says electrophoresis run is for 48 hrs. We have optimised our isolate plugs for 19 hrs. How do I synchronize it now? One issue is we get 12-13 bands...
02 November 2014 5,767 11 View
Synergism along with antibiotics against antibiotic resistant bacteria!
15 September 2014 8,887 8 View
Inhibiting the drug resistant bacterial pathogens.
10 September 2014 3,076 1 View
MALDI-TOF based identification of dermatophytes
05 September 2014 8,621 2 View
Have you faced such a problem? If yes, please suggest how was the problem rectified. Its a new equipment, being used for the first time. Under programme block 1, the initial switch time is fixed...
04 September 2014 4,925 3 View
Resuspending the master stock PRIMERS
25 August 2014 3,637 5 View
What is your experience with Fanconi Anemia cases, particularly molecular genetic testing involving - 15 genes- BRCA2, BRIP1, FANCA, FANCB, FANCC, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCL, FANCM,...
24 August 2014 6,490 2 View
Inconsistency of optimum chromosome spread.
18 August 2014 4,407 13 View
Do you think phytophagous insects may serve as potential vectors of S. enterica in association with plants/ vegetables, leading to gastroenteritis in humans? Reference for biological vectors of S....
08 August 2014 7,966 3 View
efflux pump inhibitors for MDR Gram negatives
07 August 2014 8,578 3 View
A 70 year female admitted to the ICU with UTI. Report: Isolate- Serratia marcescens Susceptibility report: Aztreonam- resistant Cefazolin- resistant Cefotaxime-...
06 August 2014 1,115 9 View
What is your opinion of hypnosis and meditation in medicine/ treatment?
28 July 2014 1,556 22 View
Tigecycline (TIG) exhibits broad-spectrum activity against many Gram-positive and Gram-negative pathogens. However, clinical resistance has emerged recently and has been detected following...
28 July 2014 4,875 3 View
Mechanical cervical traction in cervical radiculopathy. I believe it can worsen the situation. Manual traction of just few minutes a day is beneficial. I am not talking about the worst last stage...
24 July 2014 5,404 10 View
PCR based typing methods such as- Ribotyping, RAPD etc.... For Enterobacteriaceae members I found ERIC-PCR to be quite good. Many Institutes do not have facilities for PFGE and MLST. Some...
23 July 2014 9,615 1 View
Approx. 25% or more of the population carry HSV-1. Its of course true, once immuno-compromised due to HIV it predisposes to HSV infections. Further, as we know resistance to HIV has been documented.
22 July 2014 2,713 6 View
For example there is documented cross protection in other viral infections: HSV-1 can interfere with the expression of Human Papillomavirus (HPV-18) genes in cells by reducing the amount of...
22 July 2014 6,375 1 View
Both dopamine (brain messenger) related!!!!
22 July 2014 8,670 16 View
T. gondii can effectively restart the immune system by production of cytotoxic T cells (kills cancerous cells)!!!!
22 July 2014 3,721 5 View
See above
16 July 2014 5,697 1 View
Crucial key points in PFGE optimising.
15 July 2014 8,563 4 View
Interesting fact- Enterobacter asburiae competes and suppresses pathogens such as E. coli O157:H7 and Salmonella spp. I found E. asburiae in salads out of couple of food poisoning cases in my lab,...
02 July 2014 8,136 1 View
