Dr. Godfred A. Menezes

19 Questions 139 Answers 0 Followers

Questions related from Dr. Godfred A. Menezes

Maintenance of PFGE electrophoresis cell during long breaks (not in use for say 3 months)!?

What is the usual recommended procedure for maintenance of the PFGE electrophoresis cell during long breaks (not in use for 3 months): 1. Clear all the buffer/water from tubings, OR 2. Flush once...

27 April 2016 3,018 7 View

Do you think Teixobactin (discovered in 2015) could be a long lasting answer for MOST of the pan resistant bacterial pathogens?

Teixobactin was discovered using a new method of culturing bacteria in soil, which allowed researchers to grow a previously unculturable bacteria now named Eleftheria terrae, which produces the...

25 January 2015 6,914 11 View

Is anyone using the NEB Lambda ladder PFG Marker?

NEB Lambda ladder PFG Marker - literature says electrophoresis run is for 48 hrs. We have optimised our isolate plugs for 19 hrs. How do I synchronize it now? One issue is we get 12-13 bands...

02 November 2014 5,708 11 View

What are the prospectives of additives for synergism along with antibiotics against antibiotic resistant bacteria?

Synergism along with antibiotics against antibiotic resistant bacteria!

15 September 2014 8,830 8 View

Has anyone worked on synergistic action of compounds such as cryptidin along with antibiotics in inhibiting the drug resistant bacterial pathogens?

Inhibiting the drug resistant bacterial pathogens.

10 September 2014 3,008 1 View

Why am I not able to change/adjust the parameters (in. switch and fin. switch time and total run time) in the power module of CHEF DRIII?

Have you faced such a problem? If yes, please suggest how was the problem rectified. Its a new equipment, being used for the first time. Under programme block 1, the initial switch time is fixed...

04 September 2014 4,875 3 View

Are buffered solution at neutral pH better than water for resuspending the master stock PRIMERS?

Resuspending the master stock PRIMERS

25 August 2014 3,576 5 View

How can I overcome inconsistency for optimum chromosome spread in conventional cytogenetics?

Inconsistency of optimum chromosome spread.

18 August 2014 4,361 13 View

Are you aware of efflux pump inhibitors (antibiotic adjuvants) for MDR Gram negatives which have entered clinical trials?

efflux pump inhibitors for MDR Gram negatives

07 August 2014 8,525 3 View

In the below case:1. What is the mechanism of resistance? 2. Is this a stably derepressed isolate? 3. What is the drug of choice?

A 70 year female admitted to the ICU with UTI. Report: Isolate- Serratia marcescens Susceptibility report: Aztreonam- resistant Cefazolin- resistant Cefotaxime-...

06 August 2014 1,059 9 View

What is your view about hypnosis and meditation in medicine/ treatment?

What is your opinion of hypnosis and meditation in medicine/ treatment?

28 July 2014 1,497 22 View

Is the resistance to Tigecycline (an expected wonder drug) sooner than we ever thought?

Tigecycline (TIG) exhibits broad-spectrum activity against many Gram-positive and Gram-negative pathogens. However, clinical resistance has emerged recently and has been detected following...

28 July 2014 4,803 3 View

Is it recommended to go for mechanical cervical traction of several hours at a stretch in case of cervical radiculopathy?

Mechanical cervical traction in cervical radiculopathy. I believe it can worsen the situation. Manual traction of just few minutes a day is beneficial. I am not talking about the worst last stage...

24 July 2014 5,347 10 View

Among PCR based bacterial typing methods which have you found quite promising?

PCR based typing methods such as- Ribotyping, RAPD etc.... For Enterobacteriaceae members I found ERIC-PCR to be quite good. Many Institutes do not have facilities for PFGE and MLST. Some...

23 July 2014 9,558 1 View

People with HSV-1 may turn immune to HSV-2. Similarly out of wildest of chances can a HSV-1 prior infected person turn immune to HIV?

Approx. 25% or more of the population carry HSV-1. Its of course true, once immuno-compromised due to HIV it predisposes to HSV infections. Further, as we know resistance to HIV has been documented.

22 July 2014 2,670 6 View

Is resistance to HIV due to HIV-specific T cell response alone or can there be any other prior viral cross protection?

For example there is documented cross protection in other viral infections: HSV-1 can interfere with the expression of Human Papillomavirus (HPV-18) genes in cells by reducing the amount of...

22 July 2014 6,314 1 View

Have you come across coincidences of schizophrenia and T. gondii infection?

Both dopamine (brain messenger) related!!!!

22 July 2014 8,623 16 View

Is induced Toxoplasma gondii infection a potential way to treat cancer in the future?

T. gondii can effectively restart the immune system by production of cytotoxic T cells (kills cancerous cells)!!!!

22 July 2014 3,668 5 View

What is your laboratory experience with Aeromonas caviae contaminating vegetables and leading to food poisoning?

See above

16 July 2014 5,640 1 View