Dear everyone, I couldn't figure out what the formula is from the method on the paper. Artifically added pure standard must be subtracted, but I don't know how to do it. Let me briefly show the method on the paper, which I read.
[Brief method]
1. Stock solution in methanol of 1mg/ml
2. Mixture of standard solutions prepared by volumetically dilution of the stock solution with acetonitrile to 10ug/ml
3. Spiking the mixture of standard solutions of 50ug/kg
4. 2.5g of solid processed meat products weighed into a 50mL polypropylene tube
5. Internal standard(9ug/kg sample) and pipe colic acid(1mg/kg sample) in acetonitrile
6. QC samples spiked with 1, 3, 30ug/kg
7. adding 7.5mL acetonitrile with 1% formic acid
8. centrifugation then extracting 15ml supernatant
9. centrifugation then evaporation under nitrogen gas to a 0.25ml
10. adjusting volume to 1ml by adding water and put into a 0.45um filter vial
[My trial solutions]
1) The standard solution has a concentration of 50ppb
2) The original volume of unknown sample solution is 15ml(Acetonitrile 7.5ml + 1% formic acid 7.5ml)
3) The total amount of analyte in the 15mL solution is
= 15ml * (50ug/kg) * (1kg/1000g) * (g/ml) = 0.75ug
And then....I have no idea....
I tried to calculate, but there are no quantifier/qualifier ion ratios in this paper.
Please help me, please.
The weight of the sample must include in the calculation equation because you related the amount of analyte divided by weight of sample
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