11 Questions 7 Answers 0 Followers
Questions related from Seulki Choi
I have no idea why my orthotopic mouse models have failed. I have used gastric cancer cell lines, and I injected the cells into a mouse stomach. Here is my procedure and a result photo as a failed...
24 April 2024 1,885 9 View
1. tumor cell stock : 2.8 x 10^6 cells per 1 mL (Cell concentration = 2.8 x 10^6 cells/mL) 2. mice number : 2 mice 3. tumor cell number and volume when doing injection to a mouse: 1 x 10^6 cells...
08 December 2023 9,563 1 View
Dear biology scientists, Hello, I should do transduct lentivirus in the 293FT (HEK293) cells soon. However, I have failed tranformation because the DH5a colonies were too low. Let me tell my...
04 November 2023 2,173 6 View
Hello, all! I have calculated the percent of tranduced cells (%) and virus titer (TU/mL). I computed them after checking antibiotic selection process, which is a kind of functional titer, for 2...
17 June 2022 8,884 0 View
Dear all, Hello, I have just prepared the cell media, consisting of DMEM/F12, 10% FBS, and 1% penicillin-streptomycin. However, I have noticed something in my media, and I have attached a video...
02 March 2022 3,302 4 View
Dear those who have experience on ARPE-19 cell experiment, Hi, I have been culturing ARPE-19 cells. However, when I put the cells and the media in an incubator, something were observed in the...
17 February 2022 3,954 3 View
Hello, As you read the title, I prepared 344.44 mM edaravone in DMSO in the room temperature. Would you tell me whether the edaravone powder is completely dissolved? I can show you a picture on...
25 November 2021 4,363 3 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
02 March 2021 2,491 2 View
Hello, I have a question how to make the 4ppm standard mixture, and I should prepare it by mixing 205 pesticide standards in a bottle. I have looked up a method to produce the 4ppm standard, and...
15 September 2019 6,793 4 View
Hello, I'd like to ask how to interpret the results of Dunnett's test and Games-Howell test in R studio.(I attached result files of them.) I checked whether the data has equal variances among...
23 January 2018 2,052 3 View
Dear everyone, I couldn't figure out what the formula is from the method on the paper. Artifically added pure standard must be subtracted, but I don't know how to do it. Let me briefly show the...
16 August 2017 5,725 1 View
