Anyone have experience co-culturing cells that require different media?

31 May 2023 1 9K Report

I'm looking at co-culturing neutrophils and myoblasts, along with co-culturing of the conditioned media but these cells require different media.

Michael Daws

Have you tried culturing the cells in each medium to see if they will survive in one or the other? Alternatively, make a mix of equal parts of the two media and see how they survive. If neither of these works, collect a bunch of different media and test survival of both cell types in each one using small-well plates. The only way to figure this out is by empirical testing. Good luck :-)

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