Eg. do you have to start isolations immediately after blood draw? Details on collection? Does the blood have to be transported on ice or left at RT? Using combination of Histopaque and Dextran for isolations so any advice would be appreciated!
I am prepping whole blood for flow cytometry analysis of leukocytes and using BD Red Blood Cell lysis buffer (15m mins then spinning off), but my RBCs don't seem to lyse. The solution remains a...
29 October 2023 8,097 3 View
Does anyone have any suggestions or protocols for visualizing isolated neutrophils from whole blood? Just a basic morphology stain perhaps?
17 August 2023 7,827 2 View
Trying to calculate how much blood I would need to draw in order to get a sufficient number of neutrophils (from which extracellular vesicles will be obtained).
05 June 2023 5,739 2 View
I'm looking at co-culturing neutrophils and myoblasts, along with co-culturing of the conditioned media but these cells require different media.
30 May 2023 9,186 1 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
I want to draw ph diagram using Engineering equation solver EES for refrigerant R134a and R1234fy and some other. I don't know how to draw the multiple ph diagram on same dome or overlapping to...
30 July 2024 647 7 View
Please help me in plotting the photon-magnon coupling plot as shown below
14 July 2024 1,632 1 View
I heard that as the value of "num_iter(tag in wannier 90)" is higher, spread of Wannier function(=WF) is gradually lower in wannier 90. If so, is this procedure that minimize the spread of WF...
13 July 2024 4,608 0 View
Hi everyone hope your all doing good. I’ve been looping my scripts and am trying to take a cut line of the conduction band energy with respect to y for each loop. Basically, I want to take a...
03 July 2024 9,134 1 View
My feeling is that there is - but where does one draw distinction.
01 July 2024 3,716 4 View
I am looking for professional software to draw the physical structure of nanoparticles or vesicles, not the chemical structures for which we use ChemDraw, but the actual physical structures,...
23 June 2024 1,292 3 View
I have ChemDraw 22.2.0 and I want to draw PC70BM but I just found C60 and less in the templates so, any one can help me ?
18 June 2024 3,098 3 View
Polar ice
12 June 2024 9,997 1 View