Lisha van Onselen

5 Questions 1 Answers 0 Followers

Questions related from Lisha van Onselen

Red blood cells not lysing during flow prep?

I am prepping whole blood for flow cytometry analysis of leukocytes and using BD Red Blood Cell lysis buffer (15m mins then spinning off), but my RBCs don't seem to lyse. The solution remains a...

30 October 2023 8,098 3 View

Neutrophil visualization after whole blood isolation?

Does anyone have any suggestions or protocols for visualizing isolated neutrophils from whole blood? Just a basic morphology stain perhaps?

18 August 2023 7,828 2 View

How many neutrophils do people generally get from a neutrophil isolation protocol (using Histopaque/Dextran)?

Trying to calculate how much blood I would need to draw in order to get a sufficient number of neutrophils (from which extracellular vesicles will be obtained).

06 June 2023 5,740 2 View

I am starting neutrophil isolation from whole blood for the first time and looking for any practical advice?

Eg. do you have to start isolations immediately after blood draw? Details on collection? Does the blood have to be transported on ice or left at RT? Using combination of Histopaque and Dextran for...

06 June 2023 5,486 0 View

Anyone have experience co-culturing cells that require different media?

I'm looking at co-culturing neutrophils and myoblasts, along with co-culturing of the conditioned media but these cells require different media.

31 May 2023 9,187 1 View