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Questions related from Gang Dong
We are about to recombinantly express a couple of membrane proteins in E. coli and then purify them using detergent-containing buffers. We'd like to first screen a panel of detergents on a small...
23 February 2021 5,009 3 View
We have crystallized a native protein; it lacks significant homology to any other known structures. We have been trying to crystallize the SeMet-substituted protein, but have difficulties to...
16 June 2020 6,736 1 View
We are currently trying to refine a 0.9-A resolution crystal structure of a protein. Anyone knows which software is the best one to do it? Thanks!
16 June 2020 499 0 View
Dear all, It have been reported repeatedly by many labs to make crystallisation of target proteins possible only by fusion of maltose-binding protein (MBP) to the N-terminus of their targets. We...
02 April 2020 1,542 7 View
There is a recent publication titled "In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning" (J. Biol. Chem, 2019; doi:...
06 March 2020 350 3 View
We are working on a protein that is predicted to contain a zinc finger (ZnF). Could someone share your experience working with ZnF proteins? Should we add Zn during expression in E. coli? If so,...
24 February 2020 2,320 1 View
