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We have crystallized a native protein; it lacks significant homology to any other known structures. We have been trying to crystallize the SeMet-substituted protein, but have difficulties to...
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There is a recent publication titled "In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning" (J. Biol. Chem, 2019; doi:...
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We are working on a protein that is predicted to contain a zinc finger (ZnF). Could someone share your experience working with ZnF proteins? Should we add Zn during expression in E. coli? If so,...
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