Gang Dong

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Questions related from Gang Dong

Anyone has successful experience using the "I3C Phasing Kit" from HR?

We have crystallized a native protein; it lacks significant homology to any other known structures. We have been trying to crystallize the SeMet-substituted protein, but have difficulties to...

16 June 2020 6,665 1 View

Anyone has experience in "ligation-free in vivo DNA assembly in E. coli"?

There is a recent publication titled "In vivo DNA assembly using common laboratory bacteria: A re-emerging tool to simplify molecular cloning" (J. Biol. Chem, 2019; doi:...

06 March 2020 264 3 View

How to express and purify Zinc-binding proteins?

We are working on a protein that is predicted to contain a zinc finger (ZnF). Could someone share your experience working with ZnF proteins? Should we add Zn during expression in E. coli? If so,...

24 February 2020 2,223 1 View