The most important for reliability, for me, especially when running multiple samples, was to keep the timing on each sample as close to the same as possible.

Thank you very much for your answer, yeah it seems very important to automate the same moves, same speed, same person to compare the different samples. May I ask you if you used the entire cells, or extracts cells or even the LPS extracted from the cyanobacteria to run yours tests? As this method seems to be very sensitive, all the steps is crucial isn't it?

Thank you very much for all suggestions or data you may give me to run this test. Sincerely yours. K Comte

I didn't measure endotoxin for bacterial studies. I was looking at endotoxin contaimination,which is what these tests are usually designed for. This is why they are so sensitive.

With what you are trying to do, it's probably easier to just use the cell lysate. But I have a feeling that you'll also need to do the extracted LPS and the supernatant for any free endotoxin also for completeness.

If you have any published data on the expected endotoxin level, it will help you with the dilutions of your samples. Otherwise, you will just have to try your best guess and go from there.

Hope this helps.

Thank you very much for your useful suggestions Daniel and Shen-An! It will help me a lot because unfortunately very rare publications or reports are available about this case. I will plan all steps very carefully to obtain something exploitable! Thanks again!

K Comte