For CoolCell FTS30 and CoolCell LX system, do you always fill empty wells with so called filler vials (or vials with same amount of freezing medium but no cells)?
This is recommended in their manual but I am not sure how important that is. It sounds like an annoying step if you have to do so every time.
Thanks.
Hi
I am using the CoolCell LX system for few years now. Generally, you do not need to follow the instruction. I would only recommended it if you have precious samples that may be harder to recover from freezing. Indeed the temperature decrease will be more homogeneous if you have all the wells filled with cryovials containing the same volume as your sample of interest and therefore allows for an increased cell viability after thawing.
I use it as well and i have a set of tubes filled with criopreservation solution for empty positions. I use the same fill tubes over and over for two years now without any problems.
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