Diego Raffo

6 Questions 121 Answers 0 Followers

Questions related from Diego Raffo

Does anyone has a protocol for DAPI staining of non-adherent cells?

I want to test for micoplasma a non-adherent cell line. I was wondering if someone has a protocol for cases where cells cannot be attached to a coverslip

22 March 2022 2,040 4 View

Can a binocular loupe be used for watching cell cultures?

I´ve always used inverted microscopes to follow our cell cultures. I wanted to know if a binocular loupe can be used for simple things such as evaluating plate confluence, cell adherence or...

17 September 2019 4,570 4 View

Does any one knows if the basal medium from StemPro™ Chondrogenesis/Ostegenesis Differentiation Kit is DMEM low glucose?

I have the supplement but I need to replace the medium. For what I’ve red, I believe it is DMEM low glucose without phenol red, but does someone know that for sure? Or have use DMEM low as a...

13 February 2019 4,105 5 View

Has anyone tried Benzalkonium Chloride for cleaning and preventing fungal growth at the water tray of CO2 incubator?

What I want to know, is what ar the recommended Benzalkonium Chloride final concentrations for cleaning surfaces and to add to the water tray of a CO2 incubator. Looking forward for your answers!

05 January 2016 664 1 View

Has anyone tried to grow mesenchymal stem cells from explants from umbilical cord? How long does it take to see the cells growing attached to plastic?

I`m trying to grow mesenchymal stem cells from umbilical cord tissue. I`ve read several protocols and i´m starting to do it myself. However, it has been over a week and i dont see cells attached...

20 February 2015 7,184 2 View

Automatic updates in calculated fields RedCap?

I want to generate a calculated field that uses a value from another table. If I modify the original value the calculated field doesn´t update unless I open the instrument with the calculated...

01 January 1970 1,490 2 View