Someone had problems to quantify soybean proteins from leaves using ELISA?

I am trying to quantify a peptide in soybean leaves using ELISA but I couldn't. I think I have some interferents from the extraction buffer, then I dialyzed the sample before adding in the ELISA...

04 May 2018 5,813 7 View

I am looking for new molecular methodologies to identify plants at species level. Someone could help me?

31 December 2016 1,552 6 View

Does anyone know an efficient protocol to extract proteins from soybean callus?

I would like to extract proteins from soybean callus, then I am looking for a protocol to obtain high amounts of protein. I would like to quantify a protein using ELISA methodology after the...

03 April 2015 8,875 3 View

Anyone knows a protocol to perform the extraction of intracellular enzymes from filamentous fungi?

I need to prepare a crude extract of Penicillium herguei to evaluate the activity of an intracellular enzyme. Do you think maceration is a good technique?

31 December 2013 4,937 2 View

How is possible to avoid plasmid self-ligation during cloning?

We are trying to clone a plant gene in pGEM and after the transformation, we obtained only 4 colonies and all colonies were negative in the colony PCR. All 4 colonies showed plasmid self-ligation....

06 July 2013 3,512 23 View

Could you suggest a technique for quantifying protein expression?

My peptide is a plant peptide and it's expressed in E.coli BL21

05 June 2013 3,106 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View