Fernanda Cortez Lopes

7 Questions 17 Answers 0 Followers

Questions related from Fernanda Cortez Lopes

Someone had problems to quantify soybean proteins from leaves using ELISA?

I am trying to quantify a peptide in soybean leaves using ELISA but I couldn't. I think I have some interferents from the extraction buffer, then I dialyzed the sample before adding in the ELISA...

05 May 2018 5,886 7 View

I am looking for new molecular methodologies to identify plants at species level. Someone could help me?

I would like some help about the newest methodologies used to identify plants at species level. DNA barcoding seems to be the most used methodology.

01 January 2017 1,627 6 View

Does anyone know an efficient protocol to extract proteins from soybean callus?

I would like to extract proteins from soybean callus, then I am looking for a protocol to obtain high amounts of protein. I would like to quantify a protein using ELISA methodology after the...

04 April 2015 8,944 3 View

Anyone knows a protocol to perform the extraction of intracellular enzymes from filamentous fungi?

I need to prepare a crude extract of Penicillium herguei to evaluate the activity of an intracellular enzyme. Do you think maceration is a good technique?

01 January 2014 5,052 2 View

How is possible to avoid plasmid self-ligation during cloning?

We are trying to clone a plant gene in pGEM and after the transformation, we obtained only 4 colonies and all colonies were negative in the colony PCR. All 4 colonies showed plasmid self-ligation....

07 July 2013 3,691 23 View

Anybody knows a free-download software to quantify bands of proteins in SDS-PAGE?

I need to compar the expression of my protein in different conditions...

06 June 2013 6,099 7 View

Could you suggest a technique for quantifying protein expression?

My peptide is a plant peptide and it's expressed in E.coli BL21

06 June 2013 3,191 4 View