Someone had problems to quantify soybean proteins from leaves using ELISA?

I am trying to quantify a peptide in soybean leaves using ELISA but I couldn't. I think I have some interferents from the extraction buffer, then I dialyzed the sample before adding in the ELISA...

04 May 2018 5,813 7 View

I am looking for new molecular methodologies to identify plants at species level. Someone could help me?

31 December 2016 1,552 6 View

Does anyone know an efficient protocol to extract proteins from soybean callus?

I would like to extract proteins from soybean callus, then I am looking for a protocol to obtain high amounts of protein. I would like to quantify a protein using ELISA methodology after the...

03 April 2015 8,875 3 View

How is possible to avoid plasmid self-ligation during cloning?

We are trying to clone a plant gene in pGEM and after the transformation, we obtained only 4 colonies and all colonies were negative in the colony PCR. All 4 colonies showed plasmid self-ligation....

06 July 2013 3,512 23 View

Anybody knows a free-download software to quantify bands of proteins in SDS-PAGE?

I need to compar the expression of my protein in different conditions...

05 June 2013 6,015 7 View

Could you suggest a technique for quantifying protein expression?

My peptide is a plant peptide and it's expressed in E.coli BL21

05 June 2013 3,106 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View