How to report results from a multinomial logistic regression?

I have run a multinomial logistic regression and am interested in reporting the results in a scientific journal. Would it be alright to include a model summary table with the coefficients,...

01 June 2020 4,107 12 View

Which software is best suited for conducting Life Cycle Analysis (LCA) on agricultural produce in India?

I am a student planning to use LCA on agricultural products in India. There are multiple options for LCA software, but either the software itself or the supporting database is expensive. However,...

10 February 2020 342 9 View

Would monoclonal antibody aggregates effect the absorbance during a Bradford Assay?

I have purified an IgG from cell supernatant using a Protein G column. Following this size exclusion chromatography was used to determine aggregates of the IgG, monomeric antibody and antibody...

08 November 2019 1,487 3 View

Amino acid numbering: first amino acid of the mature or nascent protein?

Hi, Could anyone chime in on amino acid numbering for proteins with a secretory signal? Should the numbering start from just after where the SS gets cleaved off, or from the first methionine? I...

31 May 2017 5,405 5 View

QPCR melting curve analysis shows a shoulder on BOTH sides of the curve. What does this mean ?

11 January 2017 2,475 4 View

Why is the background signal on my Western blot not improving?

I have been performing a Western blot where I probe for my protein of interest, then strip and reprobe for the loading control (I haven't been blocking after stripping, just incubating in primary...

13 May 2015 7,484 17 View

Should I ligate my two DNA fragments together first and then ligate that construct into my plasmid? Or throw all three together in at the same time?

I have two fragments one is 2.9kb the other is 2.7kb. I need to ligate those two fragments together and then ligate my new construct into a 4.36kb plasmid. Should I do two separate reactions, one...

16 March 2015 8,755 13 View

Is there a SV40 large T-antigen encoding region for sale already ligated into a plasma that I could purchase somewhere?

I am trying to create a construct consisting of the GnRH promoter region from a chicken and the SV40 large t-antigen (TAg) encoding region for my master's thesis (to later create an immortalized...

05 March 2015 2,846 2 View

Stats - how to compare two things for significance to see if one is more valid than the other?

I'm comparing two adherence diary designs to an objective measure (an accelerometer - all will measure how much participants walk each day) and I want to assess how closely each diary relates to...

11 September 2014 4,176 6 View

Has anyone used the phospho S6 ribosomal protein antibody for western blotting in vitro?

I am trying to find out if people use the Ser 235/236 site or the Ser 240/244 site for the S6 ribosomal protein antibody? I am working in vitro using skeletal muscle and need to decide which...

01 April 2014 6,016 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View