-growing a cell line and treating with strep intermedius
-looking for a rough protocol or tips
I usually infect cells either epithelial cells or macrophages at MOI 10. bacteria that I used are Staph, Salmonella, and E.coli.
Does anyone have experience with parcitism of Chlamydomonas? We have a highly infectious parasite in our lab and can't find much information on it. I've attached some images.
11 June 2024 9,638 2 View
Review an article and develop a write up about how one can reverse chemically or physically retrogradation or stalling of starch based products such as cooking bananas
17 February 2024 5,171 0 View
Comparing 1960's gangs (Richardson gang / Kray twins) to the approx. 250 gangs in London today. motives aspirations risk heirarchy etc
16 October 2023 6,172 0 View
Good afternoon everyone, We're measuring senescence with flow cytometry (with C12FDG), we compare irradiated cells (2 Gy and 5 Gy) and non-irradiated cells (= Control). But we don't see any...
14 September 2023 8,761 4 View
Hi there, I am comparing the abundance of a protein in hypoxia (5% oxygen) versus normoxia (21% oxygen). I am assessing changes in my protein via western blot, and I used GAPDH as a loading...
29 August 2023 3,268 3 View
I would like to understand how much of a DV is explained by a number of IVs. My data set is relatively small = approx 300 in total. The data was collected at three time points so its...
28 August 2023 8,513 5 View
Female child age 9 - approaches to ongoing work and assessment sought
18 May 2023 9,163 0 View
Hi everyone, I just began running my western after loading my lysate samples, and 20mins into running the gel some of my samples look like this.... (see attached graphic). Some additional...
05 January 2023 1,377 2 View
Self efficacy and GBV
20 July 2022 2,471 0 View
I am hoping to source an archive of laryngoscopy images for a project relating to the Cormack and Lehane classification system. Are there any accessible resources for this?
26 February 2022 7,835 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View