How to report results from a multinomial logistic regression?

I have run a multinomial logistic regression and am interested in reporting the results in a scientific journal. Would it be alright to include a model summary table with the coefficients,...

01 June 2020 4,107 12 View

Which software is best suited for conducting Life Cycle Analysis (LCA) on agricultural produce in India?

I am a student planning to use LCA on agricultural products in India. There are multiple options for LCA software, but either the software itself or the supporting database is expensive. However,...

10 February 2020 342 9 View

Would monoclonal antibody aggregates effect the absorbance during a Bradford Assay?

I have purified an IgG from cell supernatant using a Protein G column. Following this size exclusion chromatography was used to determine aggregates of the IgG, monomeric antibody and antibody...

08 November 2019 1,487 3 View

Amino acid numbering: first amino acid of the mature or nascent protein?

Hi, Could anyone chime in on amino acid numbering for proteins with a secretory signal? Should the numbering start from just after where the SS gets cleaved off, or from the first methionine? I...

31 May 2017 5,405 5 View

QPCR melting curve analysis shows a shoulder on BOTH sides of the curve. What does this mean ?

11 January 2017 2,475 4 View

Why is the background signal on my Western blot not improving?

I have been performing a Western blot where I probe for my protein of interest, then strip and reprobe for the loading control (I haven't been blocking after stripping, just incubating in primary...

13 May 2015 7,484 17 View

Should I ligate my two DNA fragments together first and then ligate that construct into my plasmid? Or throw all three together in at the same time?

I have two fragments one is 2.9kb the other is 2.7kb. I need to ligate those two fragments together and then ligate my new construct into a 4.36kb plasmid. Should I do two separate reactions, one...

16 March 2015 8,755 13 View

Is there a SV40 large T-antigen encoding region for sale already ligated into a plasma that I could purchase somewhere?

I am trying to create a construct consisting of the GnRH promoter region from a chicken and the SV40 large t-antigen (TAg) encoding region for my master's thesis (to later create an immortalized...

05 March 2015 2,846 2 View

Stats - how to compare two things for significance to see if one is more valid than the other?

I'm comparing two adherence diary designs to an objective measure (an accelerometer - all will measure how much participants walk each day) and I want to assess how closely each diary relates to...

11 September 2014 4,176 6 View

Has anyone used the phospho S6 ribosomal protein antibody for western blotting in vitro?

I am trying to find out if people use the Ser 235/236 site or the Ser 240/244 site for the S6 ribosomal protein antibody? I am working in vitro using skeletal muscle and need to decide which...

01 April 2014 6,016 3 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

I need recommendations on banana fiber extraction process by retting?

Extraction of banana fiber from psuedo stem

19 January 2021 883 3 View

During my work in the laboratory?

During my work in the laboratory, I noticed that fructose, starch and dextrin did not give positive results with their statements such as iodine and sulwanof when prepared 3 to 5 days ago. Why?

25 December 2020 8,390 5 View

Why the taste of foods(item) changes with the sources of fuel we used(bio fuel,wood,dried dung) reality or meth ?

As my experience and many consumer experience they feel the different taste of the same item served cooked in a different source, is it real or not, what is the reason behind this.

24 December 2020 6,476 3 View

What's a cost effective way to make starch based nanocomposite hydrogels in large scale?

starch based nanocomposite hydrogels

13 December 2020 431 4 View

What are the best polymers that can use to produce a cooking pan which should be only made up with polymers?

Help this

30 November 2020 1,065 5 View

Zeta Potential measurement of starch solution?

I want to measure the charge on the particles in the solutions using zetasizer, but the experiment needs R.I and absorbance co-efficient to be conducted, but i cant find the stated valued for the...

08 November 2020 3,691 9 View

What is the most reliable method for starch digestibility analysis in beans?

I found papers cited Englyst et al. (1992) and mentioned Megazyme kit, But unfortunately these were not clear and hard to follow. Thanks in advance,

23 September 2020 1,476 5 View

How to rank data and transform responses of a variable in SPSS?

So i have data something like this in SPSS at the moment: case 1: orange, bananas, mangoes case 2: bananas, oranges, mangoes So the first respondent ranked orange as best fruit whilst second...

07 September 2020 8,305 5 View