Clara Lubeseder-Martellato

5 Questions 7 Answers 0 Followers

Questions related from Clara Lubeseder-Martellato

Which antibody for mouse p300 IP?

Which is the best Antibody to perform IP of endogenous mouse p300 IP. Please provide a figure or a citation in your answer. Thank you!

09 September 2019 4,286 2 View

Which is the most likely kind of cellular respiration a cell line will use when switching culture conditions from 37°C to 4°C?

In my particular case I observed an energy-dependent mechanism in my clone of interest also when incubating the cells at 4 °C (30min) as a control. The experiment was still done several times. I...

10 October 2013 9,805 1 View

Can anybody tell me the best B-raf antibody (anti-mouse B-Raf) suitable for immunofluorescence?

I need to stain B-Raf in PFA-fixed cells and tissue explants

09 September 2013 7,035 1 View

Endosomes quantification

What is the best method to quantify early endosomes in cell lines and/or animal tissue. Is the total amount of EEA1 and RAB5 in addition to quantification of EEA1+ vesicles from immunoflurescence...

12 December 2012 4,182 9 View

Endosomes isolation

Can anyone recommend a method to isolate early endosomes from animal tissue without using ultracentrifugation?

12 December 2012 6,794 4 View