We have been using the above Western standard for Western blots but the standard never shows up after visualization with a chemiluminescent substrate (except in 1 or 2 cases actually). Has that happened to anyone else too? Any suggestions?
did you add the StrepTactin-HRP or StrepTactin-AP conjugate with your secondary antibody incubation before using the chemiluminescent substrate?
Hello, I would like to know if someone could advice me about the best device to use to measure artificial light at night in coastal areas, outside and inside water (just few meters)? Thank you...
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I am pursuing my PhD and chose the topic of pros/cons and ethical concerns in leveraging Artificial Intelligence tools to increase Equity in Performance Management/Appraisal and did not find much...
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I want to calculate the ID/IG ratio from a carbon sample measured by Raman. After obtaining the spectra, I have deconvoluted D and G peaks by fitting them with 2 Gaussian-Lorentzian curves using...
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Hello Can I put a html file (a network graph) ?
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Currently I'm struggling with choosing between various analysis options, ranging from repeated measures design, to 4-way ANOVA to ANCOVA or to moderation analysis with the PROCESS macro of Hayes....
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29 September 2022 4,851 2 View
I have some polar transformed data that I would like to represent in a graph. I was used to do that in Statistica; however, I cannot find a way to do it in SPSS. Does someone know if/how to do it...
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For one of my studies, I am using Q methodology for the first time. I am currently developing the Q-survey that will be administered to participants on an online platform (I am considering using...
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I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
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I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
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Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
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I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
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I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
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How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
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Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
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A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
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Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View