Nathalie Plundrich

5 Questions 13 Answers 0 Followers

Questions related from Nathalie Plundrich

Has anyone noticed high or uneven fluorescence background with iBlot PVDF membrane stacks?

I was wondering if anyone else has used the iBlot PVDF membrane stacks and noticed uneven and/or relatively high autofluorescence / background when used for fluorescence Western blotting? I am...

30 May 2016 8,097 2 View

Protein sample (reduced, denatured, frozen) turns brown with increased storage time. Why is that?

My samples contain protein and likely green tea compounds. After my sample preparation I added NuPAGE reducing agent (Novex) and Novex NuPAGE sample buffer and heated the samples for 2 min at 85...

30 October 2014 2,344 4 View

Can/do polyphenols "migrate" on SDS-PAGE/PAGE when previously bound to protein?

Can/do polyphenols "migrate" on SDS-PAGE/PAGE when previously bound to protein? Or will they likely be broken off when sample buffer (and/or reducing agent) is added to sample and will not "be...

24 January 2014 5,120 5 View

Any suggestions for Bio-Rad Precision Plus Protein WesternC Standard not showing up on Western Blot?

We have been using the above Western standard for Western blots but the standard never shows up after visualization with a chemiluminescent substrate (except in 1 or 2 cases actually). Has that...

20 December 2013 7,816 1 View

Qualitative ATR-FTIR for evaluation of conformational modifications. Peak at ~1750 cm-1?

I am working qualitatively on an ATR-FTIR spectra of a high protein/high fat flour-majorly I am looking at modifications of Amide I and Amide II bands. Does anyone know what the band at around...

26 June 2013 7,538 19 View