7 Questions 14 Answers 0 Followers
Questions related from Nathalie Plundrich
I was wondering if there is a general consensus as to which marker is more accurate and/or suitable at reflecting mast cell degranulation in vitro? I've read that beta hex is more accurate and...
10 August 2016 8,010 7 View
I was wondering if anyone else has used the iBlot PVDF membrane stacks and noticed uneven and/or relatively high autofluorescence / background when used for fluorescence Western blotting? I am...
30 May 2016 8,166 2 View
My samples contain protein and likely green tea compounds. After my sample preparation I added NuPAGE reducing agent (Novex) and Novex NuPAGE sample buffer and heated the samples for 2 min at 85...
30 October 2014 2,393 4 View
Can/do polyphenols "migrate" on SDS-PAGE/PAGE when previously bound to protein? Or will they likely be broken off when sample buffer (and/or reducing agent) is added to sample and will not "be...
24 January 2014 5,179 5 View
We have been using the above Western standard for Western blots but the standard never shows up after visualization with a chemiluminescent substrate (except in 1 or 2 cases actually). Has that...
20 December 2013 7,863 1 View
I have a random question about the EZQ protein assay. I just used it for the first time and really like it, it suits my test purposes well, as I have samples that contain pigments and phenolic...
29 November 2013 1,069 0 View
I am working qualitatively on an ATR-FTIR spectra of a high protein/high fat flour-majorly I am looking at modifications of Amide I and Amide II bands. Does anyone know what the band at around...
26 June 2013 7,579 19 View
