I plan to work with EL-4 cell line, previously on culturing Jurkat e6.1 i found the cell were dying on sub-culturing. So i would need suggestions for optimum growth of EL-4. I have heard addition of BME helps. But what should be the amount that should be added in a 20 ml culture.
Also what stimulant should be used to activate its t cell response.
Is PMA(10 ng/ml) and inonomycin(500 ng/ml) a good stimulant?