Sneha Ghosh

4 Questions 7 Answers 0 Followers

Questions related from Sneha Ghosh

Culturing of PANC-1 cell line?

I have ordered PANC -1 from Atcc. Upon plating them, the cells are taking around 4-5 days to reach 60% confluency. However, by end of 5th day , the background is becoming blurry with what seems...

16 March 2020 10,139 3 View

Any suggestion for EL 4 culture and stimulation?

I plan to work with EL-4 cell line, previously on culturing Jurkat e6.1 i found the cell were dying on sub-culturing. So i would need suggestions for optimum growth of EL-4. I have heard addition...

06 April 2018 7,355 3 View

Pegylated liposomes are forming precipitate on trying to suspend in PBS. How can i get a clear solution of the liposomes?

I am dissolving the lipids in chloroform : Methanol (2:1) and drying the it to form a thin film, which is followed by overnight drying in vacuum. However next day when the liposome is re-hydrated...

11 December 2017 8,259 4 View

PD-1 checkpoint blockade in vitro

Does any one have any specific papers of blocking the PD-1 and PD-L1 interaction using specific antibodies in vitro on cell lines? Or papers which show the reversal of the blocking on treatment in...

01 January 1970 3,389 3 View