Protocol for fixing and mounting fungi from agar culture?

I'm looking for a good protocol for taking fungi (mostly conidial ascomycetes) growing on an agar plate and fixing them and ultimately staining and mounting them. Would one simply remove a plug of...

03 June 2019 8,227 5 View

Gelatin as a gelling agent for fungal growth media?

I'm planning an experiment involving imaging cultures of mycoparasitic Trichodermium interacting with various species of host fungi and am thinking of various methods of imaging the interaction...

26 May 2019 2,466 3 View

How to alter dimer-monomer equilibrium in proteins?

I have a protein forming a very stable dimer in solution, but still running as monomer in SDS page. I have tryed to disrupt the dimer with Beta-ME and high salt, but it is still running as dimmer...

21 January 2018 2,921 3 View

Can a short burst of electricity cause irreversible membrane damage to Ecoli strains?

No growth observed on plates and I can't explain why as the sample was only subjected to current for a second.

10 November 2017 4,016 1 View

Experience with Foxp3 intracellular staining?

Hello. Need help with (eBioscience) FoxP3 kit. I heard the external antibodies (CD4 and CD25) can be added, AFTER the fix/perm, after overnight incubation at 4C, CONCURRENTLY with the Foxp3...

03 January 2017 8,353 5 View

How much IL-10 secreted from 1million (isolated) CD4+ cells?

How much IL-10 can be expected to secreted from 1million (freshly isolated and magnetically seperated, human) CD4+ cells, stimulated for 24 and/or 48 hours 1ug/ml plated CD3+ 2ug/ml soluble...

20 December 2016 1,837 2 View

Anti-CD3 for stimulation is displaced?

I have been activating T cells with anti-CD3 (either soluble or plate bound) for many years. Currently, I am establishing a protocol whereby at the time of coating (or subsequently) I plate bind...

19 October 2016 4,989 1 View

I am getting different results from DLS measurements , what kind of errors I might have or what can be the reason ?

09 June 2016 2,614 5 View

How can I transform a data series with negative, zero, and positive data points?

The data series is from changes in bee hive weights per day, so all data points are important. They are not normally distributed (many zeros) hence the need to transform the series. I wanted to...

01 June 2016 7,183 7 View

What are the best resources to aid in determining what questions to ask in a survey?

Survey resources focus on what surveys are and how they are constructed but does not provide guidance on how or what to translate from literature review or other research inputs into a survey. Are...

10 January 2016 7,384 10 View

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02 March 2021 3,060 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View