CREB and p-CREB quantification?

I've come across a few publications were the authors to demonstrate phospho-CREB increase shows only the WB band of p-CREB without showing the total CREB. Is it correct ? because I always thought...

11 December 2016 1,557 3 View

Any suggestions in finding a Lab Host for TALENTs (European) grant?

07 August 2015 8,925 3 View

Which method can I use for protein precipitation from cell culture media and ELISA?

I'm trying to concentrate cell culture supernatant to evaluate levels of IL-1B trough ELISA. I would like to do with TCA-Acetone method. Do you have any good protocol? Also, I've read that...

03 April 2015 9,143 6 View

Hypertetraploid cell line, is possible to do gene editing?

Hi to all! I'm entering the field and so I have a lot of question..The first one is the model: We are currently working with a cell line that is Hypertetraployd , and I'm wondering if it could be...

09 October 2014 5,372 7 View

How can I solve my BglII site destruction problem?

Hi all, I need to excise a sequence from a plasmid, but this sequence was cloned in a BglII site with BamHI sequence, destroying the BglII recognition sequence. Unfortunately, the new BamHI is...

02 March 2014 5,245 10 View

Can someone recommend the easiest way to activate Nf-kB in a cell line (simply HeLa)?

I guess serum-starvation is the simplest option, but for my project is not the best idea. Any other suggestions? Thanks in advance

06 July 2013 9,511 11 View

Intronic miRNA

I'm quite new in the field, and I'm looking for a software or (better) an online platform to analyze intronic sequences in my gene-of-interest, to discover if there are potential miRNA inside the...

31 December 2012 2,453 4 View

Human Microglia Cell Line

I'm looking for a human microglial cell line, any suggestion? I've read about the HMO6 cell line, produced by Nagai et al., but I cannot find if they are commercially available or not.

31 December 2012 9,587 55 View

Can I store the excised lane from the Blue Native Gel overnight? In which buffer? Is there any risk of protein diffusion?

My problem is that in theory, I have to make the first dimension (native) run and the second dimension (SDS-page) run on the same day, and then transfer on membrane still on the same day. Bu also...

11 December 2012 404 5 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View