Can anybody tell me what are all are the antibiotics disc we can use for Antibiotic sensitivity test against Listeria species? or specifically against Listeria monocytogenus?
The CLSI document M45 suggests only MIC testing (i.e. dilution testing or MIC stripes) but not disc testing for Listeria monocytogenes. Three antibiotics are listed: penicillin and ampicillin (≤2 ug/mL susceptible) and trimethoprim-sulfomethoxazole (≤0.5/9.5 ug/mL susceptible). L. monocytogenes should be susceptible to penicillin and ampicillin but is intrinsically resistant to the cephalosporines. The EUCAST, however, provides zone diameter breakpoints for benzylpenicillin (disc Content 1 unit ≥ 13 mm susceptible), ampicillin (2 ug ≥ 16 mm susceptible), meropenem (10 ug ≥ 26 mm susceptible), erythromycin (15 u ≥ 25 mm susceptible) and trimethoprim-sulfomethoxazole (1.25/23.75 ug ≥ 29 mm susceptible).
http://www.eucast.org/clinical_breakpoints/
You should follow CLSI or EUCAST guidelines for antimicrobial susceptibility testing. Some of the antibiotics used for suspected bacterial infections are ampicillin, gentamicin and trimethoprim.
Mostly micro broth dilution method was preferred for determining the antibiotic sensitivity against L. monocytogens. It is always better to follow CLSI procedure which is quite simpler also.
It is found to be sensitive to Dual drugs treatment like Ampicillin and Gentamycin (or) amoxicillin and vancomycin, which might be useful for you to perform the experiment.
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