hello,
I have a 5`-3` forward and reverse primers and probe sequences for sul1 gene (sulfonamide resistance genes), and i want to prepare a positive control as a plasmid that be appropriate to the primer and probe
thank you all
Ammar
hi Artur
the question : i have the sequence of the primers and the probe, i got it from article and i want to prepare a Standard Curve.
for that :
i want to sent a sequence that i don't have to sequencing company for cloning plasmid that will be contain the sequence that can suitable for the primers and the probe that i have - i don't know how i can prepare this sequence
thank you a lot
thank you a lot Artur
i sent to the authors
in the option of synthesise, i want to sent the oligo, how i can prepare the suitable oligo that contain the sequences of the primers and the probe? i will have to sent a sequence of nucleotide to the synthesize company, how i can prepare it, if you know
thank you
ammar
Why don't you first discuss this issues with your supervisor who assigned this project to you or with your lab colleagues?
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