Log phase of E.coli?

hii all, i am culture a E.coli bacteria with LB Broth, what the Appropriate condition that i can growth the E.coli (temperature, pH, Shaking, time) ? and how i can ensure that i have a E.coli in...

04 May 2018 1,482 3 View

Antibiotic resistance genes in wastewater ?

i am work with antibiotic resistance genes in wastewater with Real Time PCR analysis, like Tetm gene, Sul1 gene, Intl1 gene. who can help in some questions ? thank you Ammar

31 December 2017 7,501 2 View

Real Time Pcr?

hii everybody, how i can convert my Ct result that i got from Real time PCR to copy number that i can compare with CFU\ml result ? thank you very much Ammar

08 September 2017 7,773 0 View

Gel electrophoresis (SDS-PAGE) ?

07 August 2017 3,528 13 View

Positive control as a plasmid - Real time pcr

06 July 2017 2,655 3 View

How i can know the "'gene copy number" according to the Concentration of the plasmid DNA for using as positive control on Q Real Time PCR analayize?

11 December 2016 2,257 1 View

How i can work with primer in Real Time PCR ?

Today i received a tetM primers forward and reverse and this is the first time for me to make a real time PCR reaction and i recived stock with 100(micro molar) that i need 218(micro liter) to get...

11 December 2016 3,588 3 View

Western blot for Candida Albicans ?

Hi everybody, 1) which is the best "buffer lysis" for Candida Albicans? 2) protocol for western blot that can be worked with streaked Candida albicans?

01 January 1970 5,994 4 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Does anyone have a copy of the DIBaS dataset?

I am a student from Brunel University London, and I was looking to use the DIBaS dataset for my dissertation. However, recently it has not been accessible via this link:...

26 January 2021 5,185 4 View