Log phase of E.coli?

hii all, i am culture a E.coli bacteria with LB Broth, what the Appropriate condition that i can growth the E.coli (temperature, pH, Shaking, time) ? and how i can ensure that i have a E.coli in...

04 May 2018 1,482 3 View

Antibiotic resistance genes in wastewater ?

i am work with antibiotic resistance genes in wastewater with Real Time PCR analysis, like Tetm gene, Sul1 gene, Intl1 gene. who can help in some questions ? thank you Ammar

31 December 2017 7,501 2 View

Real Time Pcr?

hii everybody, how i can convert my Ct result that i got from Real time PCR to copy number that i can compare with CFU\ml result ? thank you very much Ammar

08 September 2017 7,773 0 View

Helicobacter pylori

hii everybody, i ask about all tips that can help me to grow this bacteria

07 August 2017 6,419 8 View

Gel electrophoresis (SDS-PAGE) ?

07 August 2017 3,528 13 View

Positive control as a plasmid - Real time pcr

06 July 2017 2,655 3 View

How i can work with primer in Real Time PCR ?

Today i received a tetM primers forward and reverse and this is the first time for me to make a real time PCR reaction and i recived stock with 100(micro molar) that i need 218(micro liter) to get...

11 December 2016 3,588 3 View

Western blot for Candida Albicans ?

Hi everybody, 1) which is the best "buffer lysis" for Candida Albicans? 2) protocol for western blot that can be worked with streaked Candida albicans?

01 January 1970 5,994 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View