9 Questions 17 Answers 0 Followers
Questions related from Ammar Abou Kandil
hii all, i am culture a E.coli bacteria with LB Broth, what the Appropriate condition that i can growth the E.coli (temperature, pH, Shaking, time) ? and how i can ensure that i have a E.coli in...
05 May 2018 1,564 3 View
i am work with antibiotic resistance genes in wastewater with Real Time PCR analysis, like Tetm gene, Sul1 gene, Intl1 gene. who can help in some questions ? thank you Ammar
01 January 2018 7,552 2 View
hii everybody, how i can convert my Ct result that i got from Real time PCR to copy number that i can compare with CFU\ml result ? thank you very much Ammar
09 September 2017 7,818 0 View
hi everybody, i work with gel electrophoresis ( SDS-PAGE) , when i loading the samples with the sample buffer*4 in the wells of the gel, the samples get away and get out from the wells, the...
08 August 2017 3,576 13 View
hii everybody, i ask about all tips that can help me to grow this bacteria
08 August 2017 6,452 8 View
hello, I have a 5`-3` forward and reverse primers and probe sequences for sul1 gene (sulfonamide resistance genes), and i want to prepare a positive control as a plasmid that be appropriate to...
07 July 2017 2,720 3 View
about Real time PCR and positive control\ plasmid DNA how i can know the "'gene copy number" according to the Concentration of the plasmid DNA for using as positive control on Q Real Time PCR...
12 December 2016 2,314 1 View
Today i received a tetM primers forward and reverse and this is the first time for me to make a real time PCR reaction and i recived stock with 100(micro molar) that i need 218(micro liter) to get...
12 December 2016 3,628 3 View
Hi everybody, 1) which is the best "buffer lysis" for Candida Albicans? 2) protocol for western blot that can be worked with streaked Candida albicans?
01 January 1970 6,036 4 View
