For Saccharomyces cerevisiae, to how many cells/ml does a OD600 = 1.0 correspond to?
Hi there,
It depends on the spectrophotometer! As you measure turbidity (light diffusion in fact), the signal will depend on the geometry of your material. But roughly, 1 unit at 600nm is around 107 cells per mL( between 0.5 107 up to 2.107). The best would be to calibrate your spec by plating and counting dilutions of growing culture...
Hi there,
It depends on the spectrophotometer! As you measure turbidity (light diffusion in fact), the signal will depend on the geometry of your material. But roughly, 1 unit at 600nm is around 107 cells per mL( between 0.5 107 up to 2.107). The best would be to calibrate your spec by plating and counting dilutions of growing culture...
Hi Sai,
I fully agree with Dominique answer: roughly in the range 0.5-2.0 x 107 cells per ml depending on the spectrophotometer.
Good luck with your experiments
Philippe
Hi!
fully agree with all these answers too. Roughly in the range of 0.5 to 2 x 107 cells/ml for us too. It depends on the spectrophotometer, but also on the strain itself and on the cell culture conditions. Needs to be calibrated for every type of conditions/strain if you wish accuracy.
Good luck with your experiments
Marc
Hi there,
Yes, there is definitely a correlation between number of cells and OD600 for S. cerevisiae. The exact correlation will vary between different spectrophotometer models. For that reason, a calibration curve should be made. It can be made using the plating method, microscopy counting (using a hemocytometer) or a dead live staining (seems to be the most accurate reading).
Good luck,
Juliano
- Badges
- Science method