An appropriate and cheap method for grinding plant tissues for ELISA technique.

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Tarekegn Mengistu

Manual method is i think the cheapest technique

Alex Ignatov

There is no best method for all sample types. your choice of method should be based on experience of previous investigations. Usually, 100 mg of soft plant material (leaves) are grinded with piece of capron fiber mesh in a strong plastic bag with 1.0 ml of TPB (0.01 M potassium phosphate buffer, pH 7.4, 0.1 M NaCl, 0.1% Triton X-100 )

Kalisa Alain

There are a few inexpensive options for grinding plant tissues for ELISA without the need for expensive mashing bags. Here are a few suggestions:

Mortar and pestle: This is a traditional method of grinding plant tissues and is still widely used. Simply grind the tissue sample in a mortar and pestle until it is finely ground.

Microcentrifuge tubes: You can use microcentrifuge tubes to grind small plant tissue samples. Simply cut the plant tissue into small pieces and add them to the tube with some grinding beads. Then use a vortex mixer or bead beater to grind the tissue.

Coffee grinder: A coffee grinder can be used to grind plant tissue samples. Be sure to clean the grinder thoroughly beforehand and use only for grinding plant tissue.

Blender: A blender can also be used to grind plant tissue samples. However, be sure to add some buffer or liquid to the sample to prevent the tissue from sticking to the blades.

As for non-binding containers, you can use polypropylene microcentrifuge tubes or polyethylene bags to extract proteins for ELISA. These materials are non-binding and should not interfere with the ELISA assay. However, be sure to test the containers beforehand to ensure that they do not interfere with your specific ELISA protocol.