Dear all,
I have done polyacrylamide hydrogels for cell cultures.
For preparing the glass, I add 0.1M NaOH, heat at 100°C and then add APES/APTES (3-aminopropyltriethoxysilane). I was wondering if this prepared glass could undergo autoclaving without destroying the coating.
The idea is to go under sterile conditions after autoclaving, filtering glultaraldehyde and the rest of the protocol.
Thanks for your help!
You may try and see what happens
But you may consider to use UV light and you will end up with sterile conditions
Hi Fabien, I agree with Rafael that you might want to consider alternative sterilization methods.
The silane bound to glass surfaces can be competed off by excess H2O and OH. Just look at the chemistry of the silane bonds.
It's going to be sterile after the NaOH treatment. Can you just stay under sterile conditions after that step?
I switched form NaOH activation to plasma, so I do plasma, APTES then autoclave and the rest in sterile conditions. I just wonder if the autoclaving process can cause damage to the APTES coating. Thanks :)
Hi, is aminosilanized glass sold by any vendors (preferably Europe)? Thanks!
Usually you do it yourself instead of buying; but in any case ThermoFischer.
https://www.thermofisher.com/search/browse/results?customGroup=Silanized+Glassware#
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