How can I build one phylogenetic tree for an arthropod community when I had known their scientific names?

I am working on the phylogenetic analysis for communities of plants and arthropods. A standardized protocol of building phylogenetic tree for plant species list that was widely used in studies....

29 December 2020 181 3 View

Article of hierarchically structered porous nanofibers by electrospinning?

Hello folks I'm looking for the artice which shows difference of hierarchical structures between membranes and nanofibers. From introduction, I remember it kinda said: hierarchical membranes are...

15 September 2019 470 1 View

How to use mass-spec to detect covalent bonding of a dimer complex?

Hi all, I have a protein supposed to be around 20 kDa, but somehow on SDS-PAGE it runs at 40 kDa and previous literature also showed the same behavior and suggested a dimer complex. I am little...

30 July 2019 4,279 3 View

How to test the physical and chemical stability of solution or colloid?

How to characterize the stability of DMF ( N,N-Dimethylformamide ) or DMSO ( Dimethyl sulfoxide ) solution of Lead Iodide? Can you give me some advice or references? THANKS

06 June 2019 6,073 1 View

How do I use factor score coefficients to derive factor scores for a new sample?

Hi, I conducted factor analysis in Mplus to examine the suitability of an instrument, derive factors, and derive factor scores. I used Mplus to output factor score coefficients and...

12 February 2019 2,632 3 View

How to digest bacterial EPS at physiological temperature?

My bacterial biofilm tends to aggregate and agglutinate and I want to separate them at physiological temperature (25 - 30 C) in liquid. Any good suggestions of enzymes and preferably with...

28 January 2019 4,966 3 View

Topology Optimization using Abaqus with stress as constraint?

We usually use Strain Energy as objective with volume constraint in topology tasks. But if I want to obtain a lightweight structure that the stress in it does not exceed material strength, I...

18 June 2018 7,861 3 View

What is size of a rat hippocampus cell?

I am trying to building some microchannel devices currently to culture neurons, but I want to know the initial size(diameter) of the rat hippocampus cells (before they formed the axon and...

20 December 2017 4,748 3 View

What are the alternative uses of algae lipids aside from biofuel?

Initially, our paper's rationale was on the use of lipid extracted from algae (Chlorella sorokiniana) in biofuel. However, after deliberation of panel, they have decided that researching on...

28 November 2017 4,929 5 View

A good way to analyze miRNA qpcr data when only 1 reference gene(it turns out unstable by genorm stability ranking across the samples) is included?

Dear all, I'm looking for a proper way to analyze my miRNA qpcr data. The study was aim to screen 10+ miRNAs which have been reported to have strong relationship with a certain disease in the...

13 February 2017 9,642 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View