I only used 2 wells of 12-well gel for my first electrophoresis. Can I reuse the remaining wells at the end of this electrophoresis? Could the gelred dye in the gel have been affected by the previous electrophoresis? In my view, the dye molecule cannot move without binding with DNA, so I can use the free well one more time. But someone told me I should turn around the gel if I wanna use it one more time to avoid the influence on gelred dye after the first electrophoresis.
Paul Rutland
Us ethe gel again. Small dyes will move in the electric field but there should be enough in the gel to intercalate with the dna and if not you can post stain if the signal is a bit weak
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