Hello esteemed researcher, May I direct your attention to the image? I am curious to understand why there is only a minimal drop in pressure once the elution begins.
thank you.
What column, solvents, and gradients? An isocratic method, for example, should show no pressure change.
Please show the pressure reading on the y-axis. What conditions were used for the purification? Was it a gradient elution?
Also, which "minimal drop in pressure" do you mean? Do you mean the sharp, but short decrease in the beginning? That is likely from injection of your sample. If you mean the slow change of pressure during elution, that is likely because of mixing of two phases.
As Jack enquired, provide more info on the conditions of the chromatography.
Additionally, if it's only "minimal drop in pressure", that's good, isn't it? Do you prefer big drops in pressure?
Inspect your plungers, seals and check valves...Sonicate, and purge the system a bit longer with higher flow rates...negative spikes indicate stuck bubbles or malfunctioning pump components (mainly seals, plungers, or check valves)
It is not unusual to observe a change in pressure when the system moves from one mode to another, such as from "Manual Load" to "Inject" etc. Some other potential reasons for drop in pressure include change in buffer contents (such as salt concentration) and amount of sample bound to the column.
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