I'm having problem with silky thing in my Agrobacterium LBA4404 culture. I know these silky things are dead cells caused by stress, so i tried different media; LBB, YEM, YEP, however, they still produce those silky thing.
For the latest trial, I tried to increase aeration by growing in 500ml conical flask with only 30ml of 1/2 salt LBB (tryptone 10g/L, yeast extract 5g/L, NaCl 5g/L) adjusted to pH 7.0 and shaked at 220rpm 28 degree C but it still produced silky thing after 24 hours (those silky things were already slightly visible after overnight culture). The picture shows the culture in the above 1/2 salt LBB medium after 28 hrs of culturing.
Any opinions/ideas are welcomed and appreciated.
I normally use standard LB medium supplied with 0.1% glucose and my bacterial culture grow pretty good after 30h.
I think you should check the temperature whether it's stable during the incubation.
Thanks for the comment, i'll try out the media and check the temperature, hopefully it will grow normally this time.
Looks like the bacteria is lysing. Maybe you have a bacteria virus contamination. see if you can get a new bacteria stab from an outside source and redo your transformations .
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