Vikki Wong

18 Questions 121 Answers 0 Followers

Questions related from Vikki Wong

Can i store cereal leaf samples in -80C, then take out for cellulase extraction and quantification later? should i use fresh or dried leaf?

i'm trying to extract and quantify cellulase form cereal leaves using Megazyme kit CellG5. Since I have a lot of samples, can I store the leaf samples in -80C, then take out for cellulase...

09 September 2019 2,315 3 View

How to design primers for qpcr for genes with small introns?

I would like to design primers for RT-qpcr for my gene. I understand that to avoid amplification from potentially gDNA contaminant, primers designed should span across an intron so that we can...

08 August 2019 1,680 5 View

Is this GUS fluorometry protocol correct?

I would like to quantify GUS activity in rice leaf. Below are the protocol, but I have questions in some of the steps: 1) Prepare protein extraction buffer (50mM Phosphate Buffer pH 7, 0.1% SDS,...

08 August 2019 5,313 1 View

Is this Total protein extraction and Bradford protocol ok?

I'm extracting total protein from rice leaf and would like to quantify the total protein. So I would like to ask if my protocol below is ok? I wrote my questions in between the steps: Total...

08 August 2019 9,447 2 View

Linear or curvilinear regression for BSA standard curve (Bradford assay)? What is the acceptable range of R2 for BSA standard curve?

I've tried to prepare for BSA standard curve for Bradford assay, I tried to methods: Method 1: I mixed 20ul standard with 980ul Bradford reagent in 1.5ml tube, leave at room temperature for 5min,...

08 August 2019 2,952 11 View

White precipitate appears when anthrone reagent was added into glucose standard (anthrone test for cellulose), what went wrong?

I'm trying to quantify cellulose in rice leaf. i'm using nitric acetic method, followed by 67% sulfuric acid digestion, then using anthrone reagent to quantify the glucose released. My sample and...

05 May 2019 2,003 0 View

How can I prepare cDNA to send oversea for RT-qPCR?

I'm extracting RNA from plant using trizol and converting them to cDNA. I need to bring it oversea to collaborator for RT-qPCR. I understand that I can just pipette DNA or plasmid onto filter...

02 February 2019 1,990 5 View

Is this RNA good enough for RT-qPCR?

The picture shows plant RNA extracted from trizol and run on 1x TAE 1% agarose gel (loaded 5ul of RNA in each well) 110V 20m. 1) From what I understand normally 28S band should have double the...

02 February 2019 424 5 View

Agrobacterium LBA4404 keep producing silky things in broth culture, how can i solve this problem?

I'm having problem with silky thing in my Agrobacterium LBA4404 culture. I know these silky things are dead cells caused by stress, so i tried different media; LBB, YEM, YEP, however, they still...

07 July 2018 7,413 3 View

Why is my DNA brown in color? Is it DNA or is it protein?

I'm trying to extract DNA: The extraction buffer consists of Tris-HCl, NaCl, EDTA and SDS. I put 600ul of the extraction buffer for extraction using steel beads and homogenizer, followed by...

04 April 2017 6,021 4 View

Can long taq DNA polymerase be used to amplify small fragments such as 2kb and 1kb fragments?

I understand that PFU taq have proofreading activity, therefore can reduce error during amplification, therefore it is recommended to use polymerase with pfu ability for fragments that will be...

03 March 2017 1,953 5 View

Does the value of Potassium Exchange Capacity from a soil sample represents the amount of potassium available in the soil for plant uptake?

As far as i understand, CEC represents the quantitative value of total cations that can be stored in the soil. 1) If in a case that i got a value of 3.10 cmol/kg for potassium exchange capacity in...

09 September 2015 1,957 13 View

Does chlorantriniliprole have any effect on weevils (Elaeidobius Kamerunicus)?

Chlorantriniliprole is a diamide chemical that stimulates receptors in insect muscles to excessively release calcium leading to paralysis of the insect. It is good for dealing with Lepidopteran...

09 September 2015 2,466 1 View

How do I prepare soil sample for determination of Bacillus spp population?

I'm taking samples from peat soil and mineral soil. I know for analysis of chemical elements in soil, the soil needs to be dried to constant weight. But do I need to dry the soil as well when I...

06 June 2015 8,795 5 View

Is there any standard method to isolate mycorrhizas from oil palm roots?

I'm trying to find a way to isolate mycorrhizas from oil palm roots. As we all know, there are numerous other microbes in the soil, so is there any method to make sure that the isolation is...

01 January 2015 4,044 5 View

Is there any substitute for Antiform (anti-foaming agent) used for fungi cultured in a fermenter?

I'm culturing fungi in a fermenter and currently I'm using Antifoam A concentrate from Sigma which consists of 100% active silicone polymer to prevent foaming at 0.75%. Is there any other...

01 January 2015 2,638 8 View

Is there any methods to increase spore production for fungi cultured using fermenter?

I'm trying to increase spore production for a fungi that I isolated, identification still in progress. I'm culturing the fungi using fermenter at 450rpm/min and I get spore count of 10^7 (ten to...

01 January 2015 753 6 View

Where can I isolate yeast?

I'm trying to isolate yeast for further study, can I know where I can isolate it? after isolation, I'm going to grow yeast in fermenter, what is the optimal growing condition for yeast in term of...

01 January 2015 8,842 5 View