I agree, cDNA is pretty stable and should hold on for a couple of days with dry ice without any problem. But just to clarify, you are shipping DNA and not RNA? Read that your collaborator was doing RT-PCR and that has an RNA target

Yes i'm, shipping cDNA, but dry ice is not available locally, so i'm looking for alternatives

freez dry your samples in tubes and elute when you arrive

Hi Jan. Didn't know about these tubes and they seem the ideal solution. Very cost-effective and easy. Thanks

The isolated RNA Norovirus were converted in to the first strand cDNA by using Wizard Go Scrpit Reverse Transcriptase kit (Promega/ USA).

procedure of first strand cDNA synthesis : 1- Two microliter of RNA, 2µl of oligo (dT)15 Primer and 1 µl of nuclease free water were mixed and centrifuged at 1300 rpm for few seconds.

2- The mixture was heated at 70o c for 5 minutes and immediately chilled in ice water for 5 minutes and centrifuged at 13000 rpm for 10 seconds. 3- Fifteen microliter of reverse transcription reaction mix were prepared by mixing 4 µl of Go Scrip 5X reaction buffer, 4 µl of Mgcl2,2 µl of GoTaq Green Master Mix, 1 µl GoScript reverse transcriptase and 4 µl of nuclease free water mixed together. 4- Fifteen microliter of reverse transcription mix with five microliter of RNA and Oligo (dT) 15 Primer or Random Primers mix were combined. 5- Anneal: the tubes were placed in a controlled-temperature heat block equilibrated at 25°C, and incubate for 5 minutes.

6- Extend: the tubes incubated in a controlled-temperature heat block at 42°C for up to one hour. 7- Inactivate Reverse Transcriptase: the reverse transcriptase was thermally inactivated prior to amplification by incubating the reaction tubes in a controlled-temperature heat block at 70°C for 15 minutes.