How to digest brown adipose from mice?

03 April 2018 2,675 0 View

Where can i find the database of mice ligand and receptor?

I want to find the list of mice receptor ligand binding.

02 March 2018 5,996 3 View

Western and FCM have opposite result?

11 December 2016 2,208 3 View

Ex Vivo Functional Analysis of Dendritic Cells?

09 October 2016 240 1 View

Flow cytometry of NSG mice?

08 September 2016 7,113 3 View

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I want to check several genes in one single cell, What kit can i use?

08 September 2016 4,069 0 View

How to check the rearrangement of human T and B cells? If using PCR what is the primer or kit?

Read 3 answers by scientists with 1 recommendation from their colleagues to the question asked by Hyde Chen on Sep 21, 2016

08 September 2016 9,636 3 View

Human CFC-Assay: are they clones?(what are they)?

06 July 2016 4,325 4 View

Python for primer design and test?

I designed many primers and they will work as a mixture. I am not sure whether those primers bind with each other (instead of template). I try to find some soft to check the quality of those...

06 July 2016 8,443 4 View

How to made immortal leukemia cell?

A friend has a patient with a new mutation (leukemia). He want to keep this mutation to further study. So he collected monocyte from patient and want to make a immortal cell line. He do not know...

05 June 2016 1,903 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View