Where can i find the database of mice ligand and receptor?

I want to find the list of mice receptor ligand binding.

02 March 2018 5,996 3 View

Western and FCM have opposite result?

11 December 2016 2,208 3 View

Ex Vivo Functional Analysis of Dendritic Cells?

09 October 2016 240 1 View

Flow cytometry of NSG mice?

08 September 2016 7,113 3 View

No title

I want to check several genes in one single cell, What kit can i use?

08 September 2016 4,069 0 View

How to check the rearrangement of human T and B cells? If using PCR what is the primer or kit?

Read 3 answers by scientists with 1 recommendation from their colleagues to the question asked by Hyde Chen on Sep 21, 2016

08 September 2016 9,636 3 View

Human CFC-Assay: are they clones?(what are they)?

06 July 2016 4,325 4 View

No title

06 July 2016 1,131 1 View

Python for primer design and test?

I designed many primers and they will work as a mixture. I am not sure whether those primers bind with each other (instead of template). I try to find some soft to check the quality of those...

06 July 2016 8,443 4 View

How to made immortal leukemia cell?

A friend has a patient with a new mutation (leukemia). He want to keep this mutation to further study. So he collected monocyte from patient and want to make a immortal cell line. He do not know...

05 June 2016 1,903 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View